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An ELISA kit for the detection of bFGF FGF2 Rat This uses Sandwich ELISA Double Antibody and has a sensitivity of 9 375pg ml
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Image Search Results
Journal: Cell Transplantation
Article Title: c-Casitas b-Lineage Lymphoma Downregulation Improves the Ability of Long-term Cultured Mesenchymal Stem Cells for Promoting Angiogenesis and Diabetic Wound Healing
doi: 10.1177/0963689721989605
Figure Lengend Snippet: The migration and angiogenic factors paracrine activity were decreased in long-term cultured MSCs and increased by treating with c-Cbl LNA Gapmers. (A) HUVECs were suspended in EC complete medium (PC) or vehicle (NC)-, P3 MSCs-, P10 MSCs-P10 + scramble LNA Gapmers (P10 + SCR) MSCs-, P10 + c-Cbl LNA Gapmers (P10 + c-Cbl KD) MSCs-conditioned EC basal medium supplemented with 0.75% FBS, and incubated for 8 h (original magnification, 10×) ( n = 3). The tube length, nodes, and meshes in different groups counted in three fields per well were statistically analyzed by histogram analysis. (B) The VEGFA, HGF, and bFGF level in the conditioned media were assessed by ELISA assay ( n = 3). (C) The representative picture and histogram analysis of the stained MSCs migrated to the bottom surface of the upper chamber with 10× magnification in each group ( n = 3). Error bars represent the mean ± SE. # P < 0.05 compared with the NC group and * P < 0.05 versus between the indicated groups. bFGF: basic fibroblast growth factor; c-Cbl: c-Casitas b-lineage lymphoma; EC: endothelial cell; HGF: hepatocyte growth factor; HUVEC: human umbilical vein endothelial cell; LNA Gapmers: locked nucleic acid–modified antisense oligonucleotide gapmers; MSC: mesenchymal stem cell; NC: negative control; PC: positive control; SE: standard error; VEGFA: vascular endothelial growth factor A.
Article Snippet: The supernatant was absorbed and the amount of angiogenic factors was analyzed by vascular endothelial growth factor A (VEGFA) ELISA kit (BOSTER Biological Technology, Wuhan, China),
Techniques: Migration, Activity Assay, Cell Culture, Incubation, Enzyme-linked Immunosorbent Assay, Staining, Modification, Negative Control, Positive Control
Journal: European Cells and Materials
Article Title: Osteogenic extracellular matrix sheet for bone tissue regeneration
doi: 10.22203/ecm.v036a06
Figure Lengend Snippet: Fig. 3. Quantified content of growth factors, including BMP-2, VEGF-A, bFGF and TGF-β1. Growth factors were preserved after freeze-thaw cycling in liquid nitrogen (* p < 0.05, n = 6).
Article Snippet: Aliquots of the samples (n = 6 for each protein) were analysed using Quantikine ELISA kits to determine the specific concentration of collagen I (Mouse ProCollagen I alpha 1 ELISA Kit, ab210579, Abcam), BMP-2 (Rat BMP-2 Quantikine ELISA Kit, DBP200, R&D Systems Inc.), VEGF-A (Rat VEGF Quantikine ELISA Kit, RRV00, R&D Systems Inc.), bFGF (
Techniques: